BD Biosciences Accuri C6

BD Accuri C6 Flow Cytometer Specifications Table 1-3. BD Accuri C6 Flow Cytometer Specifications Laser Excitation 488 nm (rated at 20,000 hr life) 640 nm (rated at 20,000 hr life) Laser Profile 10 x 75 µm Laser Power 14.7 mW 640 nm Diode Red Laser 20 mW 488 nm Solid State Blue Laser Scatter Detection Forward (0 degrees, +/-13) Side (90 degrees, +/-13) Emission Detection 4 colors, standard optical filters • FL1 533/30 nm (e.g. FITC/GFP) • FL2 585/40 nm (e.g. PE/PI) • FL3 > 670 nm (e.g. PerCP, PerCP-Cy™5.5, PE-Cy7) • FL4 675/25 nm (e.g. APC) Optical Alignment Fixed alignment Flow Cell 200 µm ID quartz capillary Minimum Detectable Particle Size 0.5 µm Minimum Sample Volume 300 µL (in 12 x 75 mm tube) Pre-Set Flow Rates and Core Sizes Slow: 14 µL/min, 10 µm core Medium: 35 µL/min, 16 µm core Fast: 66 µL/min, 22 µm core Custom Sample Flow Rates 10 – 100 µL/min Custom Core Diameter 5 – 40 µm Recommended Sheath Fluid 0.22 µm filtered, deionized (DI) water Maximum Events Per Sample 1 million events per well Florescence Sensitivity, MESF* FITC < 150; PE < 100 Scatter Resolution Resolves human peripheral blood lymphocytes, monocytes, and granulocytes Fluorescence Linearity 2 ± 0.05% for chick erythrocyte nuclei (CEN) Fluorescence Precision ≤ 3% CV for CEN Data Acquisition Rate 10,000 events/second, maximum Power Requirements 100 - 240 VAC, 50/60 Hz Typical Power Consumption 154 VA Heat Output 240 Btu/hour maximum output Operating Ranges 15 - 30° C; < 80% relative humidity Instrument Size 11”H x 14.75”W x 16.5”D (27.9 x 37.5 x 41.9 cm) Foot Print with Fluid Bottles 11”H x 21.5”W x 16.5”D (27.9 x 54.6 x 41.9 cm) Weight 30 lbs (13.6 kg) Fluid bottle capacity 2 L sheath fluid, 2 L waste, 250 mL cleaner, 250 mL decontamination Signal Processing 24-bit datapath Computer Interface USB 2.0 Warranty 1 year