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The BIOSTAT® CultiBag STR Plus 50L disposable bioreactor packages are specially configured for cell culture applications. The integrated, automatically-controlled gas mixing system provides advanced sparger and overlay aeration. Air and CO2 are routed to Overlay. Air, N2, O2 and CO2 are routed to Sparger. Control happens automatically via a DO and pH controller. Optional soft buttons the N2 and O2 can be used to redirect gases to overlay or sparger via manual selection.